4 6 100 pe cation exchange column Search Results


abt-906 rsm step 2 oxidation  (Omnical Inc)
90
Omnical Inc abt-906 rsm step 2 oxidation
Abt 906 Rsm Step 2 Oxidation, supplied by Omnical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/abt+906+rsm+step+2+oxidation/10__1021_slash_acs__oprd__2c00288____op2c00288_si_001-205-20-26
Average 90 stars, based on 1 article reviews
abt-906 rsm step 2 oxidation - by Bioz Stars, 2026-09
90/100 stars
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ambroxol-salbe  (Anwendung GmbH)
90
Anwendung GmbH ambroxol-salbe
Ambroxol Salbe, supplied by Anwendung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/ambroxol+salbe/10__1055_slash_a___1341___7388-162-14-7
Average 90 stars, based on 1 article reviews
ambroxol-salbe - by Bioz Stars, 2026-09
90/100 stars
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7-amino-4-trifluoro-methyl-coumarin (afc)-buffer  (Biomol GmbH)
90
Biomol GmbH 7-amino-4-trifluoro-methyl-coumarin (afc)-buffer
7 Amino 4 Trifluoro Methyl Coumarin (Afc) Buffer, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/7+amino+4+trifluoro+methyl+coumarin++afc++buffer/pmc01613198-135-27-51
Average 90 stars, based on 1 article reviews
7-amino-4-trifluoro-methyl-coumarin (afc)-buffer - by Bioz Stars, 2026-09
90/100 stars
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andrographolide  (ChromaDex)
93
ChromaDex andrographolide
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
Andrographolide, supplied by ChromaDex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/Andrographolide/pmc08793851-68-0-18
Average 93 stars, based on 1 article reviews
andrographolide - by Bioz Stars, 2026-09
93/100 stars
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carbenicillin  (Chem Impex International)
95
Chem Impex International carbenicillin
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
Carbenicillin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/Carbenicillin+disodium+salt/pmc10561622-559-14-15
Average 95 stars, based on 1 article reviews
carbenicillin - by Bioz Stars, 2026-09
95/100 stars
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triacsin c biomol  (Biomol GmbH)
90
Biomol GmbH triacsin c biomol
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
Triacsin C Biomol, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/triacsin+c/10__1074_slash_jbc__m403630200-94-16-18
Average 90 stars, based on 1 article reviews
triacsin c biomol - by Bioz Stars, 2026-09
90/100 stars
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hkeb  (Biosynth Carbosynth)
99
Biosynth Carbosynth hkeb
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
Hkeb, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/Phenylmethylsulfonyl+fluoride/pmc01168802-51-8-43
Average 99 stars, based on 1 article reviews
hkeb - by Bioz Stars, 2026-09
99/100 stars
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n-1,3-dimethylbutyl-n-phenyl-para-phenylenediamine  (FlexSys Inc)
90
FlexSys Inc n-1,3-dimethylbutyl-n-phenyl-para-phenylenediamine
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
N 1,3 Dimethylbutyl N Phenyl Para Phenylenediamine, supplied by FlexSys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/n++1+3+dimethylbutyl++n++phenyl+p+phenylenediamine/us09416259-132-191-196
Average 90 stars, based on 1 article reviews
n-1,3-dimethylbutyl-n-phenyl-para-phenylenediamine - by Bioz Stars, 2026-09
90/100 stars
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rock-inhibitor (rock-i) y-27632  (Reagents Direct)
90
Reagents Direct rock-inhibitor (rock-i) y-27632
Representative HPLC chromatograms of diterpene lactones (i.e., <t>andrographolide,</t> 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.
Rock Inhibitor (Rock I) Y 27632, supplied by Reagents Direct, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/y27632/bio_rxiv__2021__11__05__467377-318-15-18
Average 90 stars, based on 1 article reviews
rock-inhibitor (rock-i) y-27632 - by Bioz Stars, 2026-09
90/100 stars
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zebularine  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc zebularine
( a ) CC cells and normal endometrial epithelial cell were treated with <t>Zebularine</t> or PBS for 72 hours, and cell growth was determined using cell counting kit-8 assay. ( b ) Morphology of CC cells and normal cell treated with Zebularine (50 μM) or PBS for 72 hours. ( c ) qPCR analysis of miR-124 expression in CC cells treated with Zebularine (50, 75 μM) or PBS. Protein expression of iASPP ( d ) and expression of indicated mRNAs ( e ) in CC cells after treatment with Zebularine (50 μM) or PBS. ( f ) Invasion (left) and migration (right) of CC cells treated with Zebularine (25 μM) or PBS.
Zebularine, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/zebularine/pmc05073231-114-7-10
Average 90 stars, based on 1 article reviews
zebularine - by Bioz Stars, 2026-09
90/100 stars
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dimethyl sulfoxide dmso  (Selleck Chemicals)
96
Selleck Chemicals dimethyl sulfoxide dmso
The role of p38 and JNK <t>in</t> <t>DUSP9</t> siRNA-induced expression of proinflammatory cytokines and COX2 as well as PGE2 in WISH cells. WISH cells were transfected with si- DUSP9 or NC for 24 h, followed by 24 h of treatment with <t>DMSO,</t> p38 inhibitor SB203580 (10 μM), or JNK inhibitor SP600125 (20 μM). RT-qPCR analysis of the expression of IL-1β , TNF-α , COX2 ( A , G ), IL-6 , and IL-8 ( B , H ). Magnetic Luminex Assays of the secretion of IL-1β, TNF-α ( C , I ), IL-6, and IL-8 ( D , J ). Western blot analysis of COX2 expression ( E , K ). ELISA analysis of PGE2 level ( F , L ). Data were shown as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01 vs. control (0), # p < 0.05, ## p < 0.01 vs. si- DUSP9 .
Dimethyl Sulfoxide Dmso, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/SB203580/pmc08836965-163-47-55
Average 96 stars, based on 1 article reviews
dimethyl sulfoxide dmso - by Bioz Stars, 2026-09
96/100 stars
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4 6 dichloropyrimidine  (Frontier Specialty Chemicals Inc)
90
Frontier Specialty Chemicals Inc 4 6 dichloropyrimidine
The role of p38 and JNK <t>in</t> <t>DUSP9</t> siRNA-induced expression of proinflammatory cytokines and COX2 as well as PGE2 in WISH cells. WISH cells were transfected with si- DUSP9 or NC for 24 h, followed by 24 h of treatment with <t>DMSO,</t> p38 inhibitor SB203580 (10 μM), or JNK inhibitor SP600125 (20 μM). RT-qPCR analysis of the expression of IL-1β , TNF-α , COX2 ( A , G ), IL-6 , and IL-8 ( B , H ). Magnetic Luminex Assays of the secretion of IL-1β, TNF-α ( C , I ), IL-6, and IL-8 ( D , J ). Western blot analysis of COX2 expression ( E , K ). ELISA analysis of PGE2 level ( F , L ). Data were shown as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01 vs. control (0), # p < 0.05, ## p < 0.01 vs. si- DUSP9 .
4 6 Dichloropyrimidine, supplied by Frontier Specialty Chemicals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+6+100+pe+cation+exchange+column/(4%2C6-Dichloropyrimidin-5-yl)zinc+pivalate/us08796283-453-5-28
Average 90 stars, based on 1 article reviews
4 6 dichloropyrimidine - by Bioz Stars, 2026-09
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Image Search Results


Representative HPLC chromatograms of diterpene lactones (i.e., andrographolide, 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Representative HPLC chromatograms of diterpene lactones (i.e., andrographolide, 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Amount of andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), and neoandrographolide (NAP) in 80% of ethanol (EtOH80) and water extracts of Andrographis paniculata leaves analyzed by HPLC.

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Amount of andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), and neoandrographolide (NAP) in 80% of ethanol (EtOH80) and water extracts of Andrographis paniculata leaves analyzed by HPLC.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Concentration Assay

Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on serum uric acid levels in hyperuricemic-induced rats. Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Not significantly different compared to normal group ( p > 0.05). c Significantly different compared to hyperuricemic group ( p < 0.05). d Not significantly different compared to allopurinol group ( p > 0.05).

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on serum uric acid levels in hyperuricemic-induced rats. Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Not significantly different compared to normal group ( p > 0.05). c Significantly different compared to hyperuricemic group ( p < 0.05). d Not significantly different compared to allopurinol group ( p > 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on xanthine oxidase activity in rat’s liver.

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on xanthine oxidase activity in rat’s liver.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Activity Assay, Inhibition

Effect of ethanol (80%) extract of A. paniculata leaves, andrographolide and allopurinol on protein expressions of renal URAT1 (A) , GLUT9 (B) and OAT1 (C) in hyperuricemic-induced rats obtained from Western blot analysis (D) . Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Significantly different compared to hyperuricemic group ( p < 0.05).

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of A. paniculata leaves, andrographolide and allopurinol on protein expressions of renal URAT1 (A) , GLUT9 (B) and OAT1 (C) in hyperuricemic-induced rats obtained from Western blot analysis (D) . Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Significantly different compared to hyperuricemic group ( p < 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Western Blot

Viability of human fibroblast-like synoviocyte (HFLS) cells after 27 h of exposure to 80% ethanol (EAP) and water (HAP) extracts of Andrographis paniculata leaves, andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), neoandrographolide (NAP), dexamethasome (DEXA), and 0.5% DMSO. Data are presented as mean ± SEM ( n = 3).

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Viability of human fibroblast-like synoviocyte (HFLS) cells after 27 h of exposure to 80% ethanol (EAP) and water (HAP) extracts of Andrographis paniculata leaves, andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), neoandrographolide (NAP), dexamethasome (DEXA), and 0.5% DMSO. Data are presented as mean ± SEM ( n = 3).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide, and indomethacin on swelling rate in MSU-induced inflammation in rat’s knee joint.

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide, and indomethacin on swelling rate in MSU-induced inflammation in rat’s knee joint.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Effect of ethanol (80%) extract of Andrographis paniculata leaves and andrographolide on MSU-induced inflammatory mediator secretion in rat’s knee joint synovial fluid: (A) cytokines and (B) prostaglandin E 2 . Data are presented as mean ± SEM ( n = 6). a Significantly different compared to MSU control group ( p < 0.05). b Not significantly different compared to normal control group ( p > 0.05). c Not significantly different compared to indomethacin group ( p > 0.05).

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves and andrographolide on MSU-induced inflammatory mediator secretion in rat’s knee joint synovial fluid: (A) cytokines and (B) prostaglandin E 2 . Data are presented as mean ± SEM ( n = 6). a Significantly different compared to MSU control group ( p < 0.05). b Not significantly different compared to normal control group ( p > 0.05). c Not significantly different compared to indomethacin group ( p > 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Control

( a ) CC cells and normal endometrial epithelial cell were treated with Zebularine or PBS for 72 hours, and cell growth was determined using cell counting kit-8 assay. ( b ) Morphology of CC cells and normal cell treated with Zebularine (50 μM) or PBS for 72 hours. ( c ) qPCR analysis of miR-124 expression in CC cells treated with Zebularine (50, 75 μM) or PBS. Protein expression of iASPP ( d ) and expression of indicated mRNAs ( e ) in CC cells after treatment with Zebularine (50 μM) or PBS. ( f ) Invasion (left) and migration (right) of CC cells treated with Zebularine (25 μM) or PBS.

Journal: Scientific Reports

Article Title: Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124

doi: 10.1038/srep35480

Figure Lengend Snippet: ( a ) CC cells and normal endometrial epithelial cell were treated with Zebularine or PBS for 72 hours, and cell growth was determined using cell counting kit-8 assay. ( b ) Morphology of CC cells and normal cell treated with Zebularine (50 μM) or PBS for 72 hours. ( c ) qPCR analysis of miR-124 expression in CC cells treated with Zebularine (50, 75 μM) or PBS. Protein expression of iASPP ( d ) and expression of indicated mRNAs ( e ) in CC cells after treatment with Zebularine (50 μM) or PBS. ( f ) Invasion (left) and migration (right) of CC cells treated with Zebularine (25 μM) or PBS.

Article Snippet: The cells were treated with PBS or Zebularine (25–100 μM, Stem Cell Technologies, Vancouver, BC) for 72 hours.

Techniques: Cell Counting, Expressing, Migration

The expression of miR-124 is silenced by DNA methylation in CC. The restoration of miR-124 via Zebularine reduces the expression of iASPP (a direct suppressor of p53), thereby activating the p53 signaling network and suppressing tumor growth, EMT, invasion, cancer stemness and metastasis.

Journal: Scientific Reports

Article Title: Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124

doi: 10.1038/srep35480

Figure Lengend Snippet: The expression of miR-124 is silenced by DNA methylation in CC. The restoration of miR-124 via Zebularine reduces the expression of iASPP (a direct suppressor of p53), thereby activating the p53 signaling network and suppressing tumor growth, EMT, invasion, cancer stemness and metastasis.

Article Snippet: The cells were treated with PBS or Zebularine (25–100 μM, Stem Cell Technologies, Vancouver, BC) for 72 hours.

Techniques: Expressing, DNA Methylation Assay

The role of p38 and JNK in DUSP9 siRNA-induced expression of proinflammatory cytokines and COX2 as well as PGE2 in WISH cells. WISH cells were transfected with si- DUSP9 or NC for 24 h, followed by 24 h of treatment with DMSO, p38 inhibitor SB203580 (10 μM), or JNK inhibitor SP600125 (20 μM). RT-qPCR analysis of the expression of IL-1β , TNF-α , COX2 ( A , G ), IL-6 , and IL-8 ( B , H ). Magnetic Luminex Assays of the secretion of IL-1β, TNF-α ( C , I ), IL-6, and IL-8 ( D , J ). Western blot analysis of COX2 expression ( E , K ). ELISA analysis of PGE2 level ( F , L ). Data were shown as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01 vs. control (0), # p < 0.05, ## p < 0.01 vs. si- DUSP9 .

Journal: International Journal of Molecular Sciences

Article Title: miR-132-3p Modulates DUSP9-Dependent p38/JNK Signaling Pathways to Enhance Inflammation in the Amnion Leading to Labor

doi: 10.3390/ijms23031864

Figure Lengend Snippet: The role of p38 and JNK in DUSP9 siRNA-induced expression of proinflammatory cytokines and COX2 as well as PGE2 in WISH cells. WISH cells were transfected with si- DUSP9 or NC for 24 h, followed by 24 h of treatment with DMSO, p38 inhibitor SB203580 (10 μM), or JNK inhibitor SP600125 (20 μM). RT-qPCR analysis of the expression of IL-1β , TNF-α , COX2 ( A , G ), IL-6 , and IL-8 ( B , H ). Magnetic Luminex Assays of the secretion of IL-1β, TNF-α ( C , I ), IL-6, and IL-8 ( D , J ). Western blot analysis of COX2 expression ( E , K ). ELISA analysis of PGE2 level ( F , L ). Data were shown as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01 vs. control (0), # p < 0.05, ## p < 0.01 vs. si- DUSP9 .

Article Snippet: To verify the involvement of p38 and JNK signaling pathways in the induction of IL-1β, IL-6, IL-8, TNF-α, and COX2 as well as PGE2 secretion by DUSP9 siRNA, the cells were transfected with NC or si- DUSP9 for 24 h, followed by 24 h of treatment with dimethyl sulfoxide (DMSO), p38 inhibitor SB203580 (10 μM, Selleck, Houston, TX, USA), or JNK inhibitor SP600125 (20 μM, Selleck, Houston, TX, USA).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Luminex, Western Blot, Enzyme-linked Immunosorbent Assay, Control